Cellular and molecular assays. (A) Cell viability as evaluated by cell counting through trypan blue staining. (B) Apoptotic induction, as determined through annexin V-specific antibody and propidium iodide (PI) staining, and followed by flow cytometry analysis. (C) Loss of mitochondria Δ_Ψm, as evaluated by rhodamine 123 and PI double staining, and followed by flow cytometry analysis. (D) Comparison between microarray (▪) and real-time RT-PCR data (♦). C indicates control; A, ATO; S, imatinib mesylate; A+S, ATO plus imatinib mesylate.