Evidence from bone marrow cells of patients with CML. (A) Apoptotic induction, as evaluated through annexin V-specific antibody and propidium iodide (PI) staining, and followed by flow cytometry analysis. (B) Real-time RT-PCR analysis of specific marker genes. Total RNA was prepared from each of the patient samples and quantitatively analyzed by iCycler iQ real-time PCR detection system (Bio-Rad) using SYBR Green I (Applied Biosystems). P1: the patient in blast crisis; P2: the patient in chronic phase. Treatment conditions are indicated in the top right corner of each panel. Ratio stands for value of treated sample versus that of untreated sample of the same patient.