Recipients of β7-/- donor T cells have significantly less gastrointestinal target organ abnormality and no significant difference in skin GVHD. Lethally irradiated (1300 cGy) B10.BR recipients underwent transplantation with 5 × 106 WT TCD BM and either 1 × 106 WT or β7-/- splenic T cells. On day 7 (A) and day 14 (B), mice were killed and gastrointestinal target organs were harvested for evaluation of histopathology, as described in “Materials and methods.” Shown are combined results of pathology scores for 10 mice in each group (*P < .001). (C) Ear and tongue were harvested on days 7 and 14 for evaluation of skin histopathology. (D) Lymphocytes per high-power field (600 ×) from organs on day 7. Shown are results of 5 fields per organ. *P < .001 (n = 10). (E) Representative photomicrographs from day-7 organs. Green arrows show typical apoptotic cells, and black arrows show typical lymphocytes. Original magnification × 600. Images were captured with an Olympus BX 40 microscope (Olympus, Melville, NY) equipped with a 10 ×/0.40 numerical aperture objective lens. Image acquisition was performed with a JVC GC-Qx 5HDU digital camera (JVC, Wayne, NJ).
