Enhanced CTL recognition of HLA-A2⁺ aECs on exposure to proinflammatory cytokines. (A) Up-regulated expression of KDR in HUVEC1 and 2 cells on treatment with TNF-α (20 ng/mL) or IL-1β (10 ng/mL) for 24 hours. KDR protein in 30 μg HUVEC1-cell lysates was determined by Western blot. The β-actin control is shown for each extract. (B) Enhanced recognition of TNF-α- or IL-1β-treated aECs by K288.E5. HUVEC1 and HUVEC2 cells were pretreated as described for 24 hours. After washing, they were cocultured with T cells at an E/T ratio of 3:1. Results are presented from 24 hours of IFN-γ production (pg/mL). (C) Enhanced specific lysis of TNF-α- or IL-1β-treated HUVEC1 cells by K288.E5 at different E/T ratios. Cytolysis was assessed in a 16-hour LDH release assay. Data shown are representative of 2 independent experiments.