Decreased allostimulation capacity of DCs cocultured with HIV-exposed stimulated T cells. DCs cocultured with autologous T cells as described in Figure 1 were irradiated (30 Gy [3000 rad]) and used to stimulate allogeneic naive CD4⁺ T cells, at the indicated ratios of DCs to T cells. As a control, allogeneic T cells were cultured alone. After 4 days, proliferation and production of cytokines was measured. (A) Proliferation. Results are expressed as simulation index (average radioactivity in wells containing DCs and T cells/average radioactivity in wells containing control T cells). Average cpm measured in wells containing only T cells was 471. Results are from 1 representative individual of 2 individual subjects tested. (B) Cytokine production. Results are expressed as mean production of IL-10 or IFN-γ (average of 3 wells containing DCs and T cells).