Figure 4.
Physical association and phagocytosis of CD61+ cells by CD11c+ cells. Five days after culture with 100 ng/mL TPO and 2.5 ng/mL TNF-α, the cells were cytospun and stained with anti-CD11c (brown) and anti-CD61 (red) (A) or with anti-CD11c (red) and anti-ssDNA (brown) (B); original magnification, × 1000. (C) Cells placed on coverslips were fix-permeabilized, stained with FITC-CD61 (green) and PE-CD11c (red), and then were observed by laser scanning confocal microscopy. A serial 3-dimensional reconstruction of the cells demonstrates the phagocytosis of CD61+ cells (green) by CD11c+ cells (red).