Figure 5.
![Figure 5. Autologous T-cell proliferation by DCs generated in the presence of TPO and TNF-α. The cells prepared by culture for 7 days with 100 ng/mL TNF-α in the presence (▪) or absence (□) of 100 ng/mL TPO were used at graded doses as stimulator cells after γ-irradiation. In some MLRs, anti-CD3 mAb was added. Proliferation was assessed by adding 3H-thymidine (1 μCi per well [37 κBq/well]) during 72 to 90 hours of culture. Results represent the mean ± SD of triplicate cultures.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/4/10.1182_blood-2005-08-3155/2/zh80040691350005.jpeg?Expires=1735552650&Signature=zeTLqmwrzVO3xl892UFKh-Gi2hBiQtQPXjkvsXL2oHapDD~kUrsY8bQw7AeDPtb3ByKKFiT5FVuAveMmmMEX6O-6BZNX9op7Ks9A5a~dgUBiFd8RuvjwNpDt1FQzeqBOtxydG6xatdSBIkVzA76LLssPY60PgFdT6GsOGlrHecLuKmTi8RX~ghWQ1Vc9mUnUOnEOWthn6H9dhYvK5IXmS-bCD9swj4dd2bnncFAgklyJFYQDxKPcMNYhW6ktaG6LHi6-Gz0qnY5jjTtA0yXHmX9EGlwp1-MJQR5iBlXKsUKllu9b-8TQwjmkx3lWYjdf9kMiW~DtNCutqJrsMoSp7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Autologous T-cell proliferation by DCs generated in the presence of TPO and TNF-α. The cells prepared by culture for 7 days with 100 ng/mL TNF-α in the presence (▪) or absence (□) of 100 ng/mL TPO were used at graded doses as stimulator cells after γ-irradiation. In some MLRs, anti-CD3 mAb was added. Proliferation was assessed by adding 3H-thymidine (1 μCi per well [37 κBq/well]) during 72 to 90 hours of culture. Results represent the mean ± SD of triplicate cultures.
