LYL8 depletion significantly increases survival and decreases the degree of GVHD over time. Lethally irradiated BALB.B mice underwent transplantation on day 0 with 5 × 10⁶ B6 TCD-BM cells and 2 × 10⁶ CD3⁺ B6 T cells. Survival was followed for 90 days and is plotted as Kaplan-Meier curves (representative of 2 combined experiments for each condition). (A) ○ indicates no T cells (n = 7); □, nondepleted (n = 9); ♦, LYL8-depleted (n = 9); ▪, HSV8 control-depleted (n = 4). Depletion was 85.6% efficient in these transplants. ♦ versus ▪, P < .001; ♦ versus □, P < .01. (B) Clinical GVHD scoring for the recipient mice of the combined transplants plotted in panel A. ♦ versus ▪, P < .01; ♦ versus □, P < .01.