Figure 4.
Figure 4. Effect of function blocking antibodies on adhesion of the αDβ2-expressing HEK 293 cells. Calcein-labeled αDβ2-expressing HEK 293 cells were preincubated for 20 minutes at 22°C with anti-β1 polyclonal antibody 1950 (1:500 dilution), 20 μg/mL anti-αD polyclonal antibody, or with combinations of anti-β1 with either anti-αD or anti-β2 mAb IB4 (20 μg/mL). Cells were added to microtiter wells coated with various ligands at concentrations that produce maximal adhesion, and cell adhesion was determined as described in Figure 3. Data are expressed as a percentage of control (adhesion in the absence of antibodies) and are the mean ± SE of 3 individual experiments performed in triplicate in each experiment.

Effect of function blocking antibodies on adhesion of the αDβ2-expressing HEK 293 cells. Calcein-labeled αDβ2-expressing HEK 293 cells were preincubated for 20 minutes at 22°C with anti-β1 polyclonal antibody 1950 (1:500 dilution), 20 μg/mL anti-αD polyclonal antibody, or with combinations of anti-β1 with either anti-αD or anti-β2 mAb IB4 (20 μg/mL). Cells were added to microtiter wells coated with various ligands at concentrations that produce maximal adhesion, and cell adhesion was determined as described in Figure 3. Data are expressed as a percentage of control (adhesion in the absence of antibodies) and are the mean ± SE of 3 individual experiments performed in triplicate in each experiment.

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