LC reconstitution by lymphoid progenitor cells. (A) Histogram plots represent CD45.1 expression profile of epidermal-cell suspensions 2 and 3 weeks after adoptive transfer of either 10⁴ CLPs or 5 × 10⁴ pro-T1 or control cells (autologous BM only). Contour plots show corresponding I-A^b/CD11c expression profile of gated CD45.1⁺ and CD45.1^- epidermal cells from mice that underwent transplantation. (B) Contour plot shows B220/TCRγδ profile of CLP-derived LCs (CD45.1⁺, I-A^b+, CD11c⁺ population) isolated from the epidermis of mice that received transplants of CLPs at 3 weeks after adoptive transfer. (C) Top contour plot shows CD45.1/CD45.2 expression profile of total spleen cells at 3 weeks after adoptive transfer of 10⁴ CLPs. Bottom contour plot represents TCR/CD19 expression profile of gated CLP-derived (CD45.1⁺) spleen cells. (D) Chart shows average LC reconstitution (% of total LCs) obtained per 10⁴ progenitor cells at 2 weeks (both myeloid and lymphoid progenitors) and 3 weeks (only CLPs, pro-T1, pro-T2) after adoptive transfer. ND indicates not determined. Data are representative of at least 5 transplantations per progenitor population with similar results.