Morphology of CLP- and CMP-derived LCs. (A) Immunofluorescence staining with anti-CD45.1-PE and anti-I-Ab-FITC of epidermal sheets prepared from mice 3 months after transplantation and UV light exposure with either 10⁴ CD45.1 CMPs or 10⁴ CD45.1 CLPs. Epidermal sheets from CD45.2 mice that received transplants of only autologous BM cells served as controls. Scale bar: 10 μm. (B) Cytospin preparations of sorted CMP- or CLP-derived LCs. Sorted CD45.1⁺/I-A^b+/CD11c⁺/B220^-/TCRγδ^- epidermal cells derived from mice that received transplants of either CMPs or CLPs at 3 months after transfer were spun onto glass slides and stained with Wright solution. Scale bar: 10 μm. (C) RT-PCR analysis of Cd207 expression in sorted CD45.1⁺/I-A^b+/CD11c⁺/B220^-/TCRγδ^- epidermal cells. c-DNA was prepared at 4 weeks after transplantation from mice reconstituted with either 10⁴ CD45.1 CMPs or 10⁴ CD45.1 CLPs. cDNAs from LC numbers used per PCR reaction are given in brackets. Actb primers acted as controls. Data are based on at least 2 experiments with similar results.