Kinetics of neutrophil rolling, arrest, and transmigration on inflamed endothelium. Isolated human neutrophils were incubated with IC487475, BIRT, TS1/22, 2LPM19c, or vehicle, as indicated. Neutrophil suspensions were then perfused into a parallel-plate flow chamber over IL-1β activated or unstimulated control HUVEC monolayers at a shear stress of 2 dyne/cm2. (A) The number of rolling neutrophils, defined as those moving more than 1 cell diameter in 30 seconds, was quantitated in a field of view every minute throughout the interaction. Data represent the mean ± SEM from 5 to 10 separate experiments. (B) The number of adherent neutrophils, defined as those moving less than 1 cell diameter in 30 seconds, was quantitated in a field of view every minute throughout the interaction. Data represent the mean ± SEM from 5 to 10 separate experiments. (C) The number of transmigrated neutrophils was quantitated in a field of view every minute throughout the interaction. Data represent the mean ± SEM from 5 to 10 separate experiments.
