Allosteric inhibition of LFA-1 diminishes persistence of migration. Isolated human neutrophils were incubated with IC487475 or vehicle, then perfused into a parallel-plate flow chamber over IL-1β-activated HUVECs at a shear stress of 2 dyne/cm². After arrest, the centroid of migrating neutrophils was tracked at 5-second intervals for 30 seconds. Arrows outline the trajectory of migrating neutrophils, with the arrowhead positioned at cell centroid at the conclusion of each time segment. Micrographs are representative of approximately 100 individual neutrophil observations over 4 to 6 separate experiments.