Figure 6.
Figure 6. CXCR4 and CX3CR1 as epitopes on MSCs. (A) Flow cytometry of unsorted MSCs with anti-CXCR4-PE. The positive cells are primarily low in FS. (B) Real-time RT-PCR assay of CXCR4 in RS-MSCSs (green), SR-MSCs (red), and unsorted MSCs (yellow). (C) Real-time RT-PCR assay of GAPDH as a loading standard. (D) Immunocytochemistry of RS-MSCs and SR-MSCs cultured in chambered slides and stained with antibody to CXCR4 and DAPI (magnification, 400 ×). (E) Flow cytometry of unsorted MSCs with anti-CX3CR1-FITC. The positive cells are primarily low in FS. (F) Real-time RT-PCR assay of CX3CR1 in RS-MSCSs (green), SR-MSCs (red), and unsorted MSCs (yellow). (G) Real-time RT-PCR assay of GAPDH. (H) Immunocytochemistry of RS-MSCs and SR-MSCs cultured in chambered slides and stained with antibody to CX3CR1 and DAPI (magnification, 400 ×).

CXCR4 and CX3CR1 as epitopes on MSCs. (A) Flow cytometry of unsorted MSCs with anti-CXCR4-PE. The positive cells are primarily low in FS. (B) Real-time RT-PCR assay of CXCR4 in RS-MSCSs (green), SR-MSCs (red), and unsorted MSCs (yellow). (C) Real-time RT-PCR assay of GAPDH as a loading standard. (D) Immunocytochemistry of RS-MSCs and SR-MSCs cultured in chambered slides and stained with antibody to CXCR4 and DAPI (magnification, 400 ×). (E) Flow cytometry of unsorted MSCs with anti-CX3CR1-FITC. The positive cells are primarily low in FS. (F) Real-time RT-PCR assay of CX3CR1 in RS-MSCSs (green), SR-MSCs (red), and unsorted MSCs (yellow). (G) Real-time RT-PCR assay of GAPDH. (H) Immunocytochemistry of RS-MSCs and SR-MSCs cultured in chambered slides and stained with antibody to CX3CR1 and DAPI (magnification, 400 ×).

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