Comparison of histopathology in control and sickle mice. The columns show microscopic findings in control (A, C, E, G) and sickle (B, D, F, H) mice. The 4 rows (top to bottom) show findings in the spleen (A, B), bone marrow (C, D), liver (E, F), and heart (G, H). (A, B) Sickle mice had severe splenic sinusoidal congestion by sickle erythrocytes, increased hematopoietic cells, infarcts (arrows), and vascular ectasia (inset) (H&E original magnification, 100 ×; inset, 400 ×). (C, D) Erythroid hyperplasia was more severe in the bone marrow of sickle mice (H&E original magnification, 100 ×). (E, F) Hepatic findings in sickle mice included more sinusoidal congestion, infarct, and siderosis (inset) (H&E original magnification, 100 ×; insets iron stain, 400 ×). (G, H) Cardiac findings in sickle mice included infarcts, which, when acute, appeared as loss of normal striations (arrows).