Comparison of histopathology in control and sickle mice. The columns show microscopic findings in control (A, C, E, G) and sickle (B, D, F, H) mice. The 4 rows (top to bottom) show findings in the lung (A, B), kidney (C, D), glomerulus (E, F), and cerebellum (G, H). (A, B) Pulmonary findings in the sickle mice included more severe vascular congestion (arrows) and siderosis (inset) (H&E original magnification, 100 ×; inset iron stain, 100 ×). (C, D) Renal findings included infarcts (arrows) and siderosis (inset) in the sickle mice (Trichrome stain original magnification, 100 ×; inset iron stain, 100 ×). (E, F) Glomerular changes in the sickle mice included mesangial hyperplasia (arrows). (Trichrome stain original magnification, 400 ×). (G, H) Cerebellar findings included pyknotic Purkinje cell in the sickle mice (arrows) (Trichrome stain original magnification, 100 ×).