Representative local responses to the autologous combination vaccine in punch biopsy specimens from the injection site. (A-D) Control tissues from healthy volunteers. (A) CD1a (O10 staining). (B) Dendritic cells (S-100 staining). (C) CD4 (CD45RO/OPD4 staining). (D) CD8 (CD45RO/C8/144B staining). (E-H) Representative tissues from patients. (E) Abundant dermal perivascular infiltration of Langerhans cells (O10 staining) with (F) retention of dendritic-cell infiltrate in patient 5 (S-100 staining). (G) Paucity of CD4⁺ lymphocytes (CD45RO/OPD4 staining) with (H) moderate infiltration of CD8⁺ lymphocytes in patient 2 (CD45RO/C8/144B staining). These studies were performed on all patients. For all panels, original magnification is × 20. Images were visualized using a Nikon Labophot-2 microscope equipped with a 20×/1.0 Plan objective lens (Nikon, Tokyo, Japan). A Spot Insight QE 4.2 camera (Diagnostic Instruments, Sterling Heights, MI) was used to capture images, and Adobe Photoshop Elements 2.0 software (Adobe Systems, San Jose, CA) was used to process them.