Figure 3.
Representative ELISPOT results showing granzyme B, IFN-γ, or IL-5 release from PBMCs obtained before or after 3 or 6 immunizations with recipient-derived leukemia vaccines. PBMCs were stimulated ex vivo against recipient-derived leukemic blasts alone (▪) or after preincubation with anti-CD4 (
) or anti-CD8 (□) monoclonal antibodies. (A) Granzyme B, patient 4. (B) IFN-γ, patient 4. (C) IFN-γ, patient 7. (D) IL-5, patient 2. All values are mean spot numbers for duplicate wells minus background per 106 PBMCs.
