Figure 4.
![Figure 4. IRp60 does not influence eotaxin-induced calcium influx. Eosinophils previously incubated with either anti-IRp60 or control antibodies (isotype, anti-p58.2) followed by the cross-linker sheep anti-mouse F(ab′)2 were washed and loaded with Calcium Green-1AM. The cells were allowed to flow freely in the cytometer until the indicated time point at which eotaxin (1-100 ng/mL) was added (↑). [Ca2+] is expressed as mean fluorescent intensity (MFI). NA indicates not activated; Etx, eotaxin activated (n = 3). Data are expressed as relative cell count ± SD and are representative of 1 of 4 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/5/10.1182_blood-2005-07-2926/2/zh80050691790004.jpeg?Expires=1735843908&Signature=ff2RNdKJYEDwANiX3wDM1lDjmeKURQ7j103yxi9pnI9V8DKB12-UO06jZNoyZvOPAC01n6FDRohQWmExaGLryKj2nMg~uOinUIVNfEpfvKCCQpBZnM2WwaSHERp8mphoe6T2AhK2y4btjpKkNCBhSiYw~POEqFa5~iZM0oBw33mTkJBE4fhieXKR7Hz0OxP0XFpYbSMeBRNIm~2TKSO3k3bOFJgVRuaQNKYqJUA2DMSFM8a7hDIEwwy4GiOc8u3WW6BaqJctAszCZoPumctv-QHkPjWjzCNZ6yIDDF8KSwym6Gp8WPIdexkI275B5uorAYlop~uVjqbHwPXMwC7~jA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IRp60 does not influence eotaxin-induced calcium influx. Eosinophils previously incubated with either anti-IRp60 or control antibodies (isotype, anti-p58.2) followed by the cross-linker sheep anti-mouse F(ab′)2 were washed and loaded with Calcium Green-1AM. The cells were allowed to flow freely in the cytometer until the indicated time point at which eotaxin (1-100 ng/mL) was added (↑). [Ca2+] is expressed as mean fluorescent intensity (MFI). NA indicates not activated; Etx, eotaxin activated (n = 3). Data are expressed as relative cell count ± SD and are representative of 1 of 4 experiments.
