MAPK dependence for cytokine production. IL-2-cultured PBLs were treated 15 minutes with vehicle, U0126 (10 μM), or SB203580 (3 μM), then stimulated 5 hours with PMA + calcimycin + IL-2 + monensin. Indicated is the percent inhibition of cytokine production by T cells, relative to cells treated only with vehicle before stimulation (n = 3).