Figure 2.
Th-cell reconstitution in MHCII-deficient mice after thymic transplantation. Thymic lobes from embryonic day 14 (e14) C57BL/6 embryos were transplanted under the kidney capsule of Aα-/-, RFX5-/-, and CIITA-/- mice. Nine weeks after transplantation, inguinal LN cells of mice that underwent transplantation and control mice were analyzed for CD4 and CD8 expression by flow cytometry. The percentage of cells in the indicated quadrants is given in the top right corner of each dot plot. The CD4/CD8 ratio was calculated for each mouse and is shown in Table 1. The results are representative of several experiments performed.