LPS induces cGK-dependent phosphorylation of VASP in DCs, which is inhibited by NOR4. iDCs were treated or not for 24 hours with 0.3 μg/mL LPS in the presence or absence of 100 μM NOR4. Cells from each group were then stimulated for the indicated times with either CCL19 or CXCL12. (A,B) Cell extracts were prepared and immunoblotted with anti–phospho-VASP antibody and then with anti-VASP antibody. (A) Antiphospho-VASP immunoblot of 1 representative experiment of 3 performed with cells from different donors. (B) Quantitative densitometric analysis of 3 different immunoblot experiments. The activated phospho-VASP expression was normalized to the total VASP. ^*P < .001, paired Student t test. (C) mRNA levels of cGKIβ determined by quantitative real-time RT-PCR. The relative expression of cGKIβ was normalized to the endogenous β-actin. Data shown are from 1 representative of 2 independent experiments giving the same results. (D) cGMP levels in cell extracts were measured with cGMP EIA assay. Data shown are representative of 2 independent experiments.