Validation of selected components of the TAM transcriptome by RT-PCR and ELISA. (A) Real-time PCR of few highly expressed genes from the TAM transcriptome. The same pool of PEC and TAM RNA used in the array analysis was subjected to real-time PCR for the indicated genes. Representative results are given as fold increases in mRNA expression with respect to (wrt) the PECs. Data were normalized to actin gene expression as mentioned in “Materials and methods.” (B) ELISA detection for CCL2, CCL5, CXCL9, CXCL10, CXCL16, and IL-10 proteins in the culture supernatants of untreated PECs and TAMs cultured overnight under standard conditions. ND indicates not determined. Data are mean ± standard deviation (SD), representative of 3 independent experiments done in triplicate (PECs vs TAMs, P < .05).