Divergent regulation of the NF-κB and IRF-3/STAT1 pathway in TAMs. (A) Laser confocal microscopic representation of IRF-3 activation in TAMs. Untreated or 2-hour LPS-treated (100 ng/mL) PECs and TAMs were stained for IRF-3 (red) or with SYTO for nuclear counter-staining (green) and visualized by laser confocal microscopy. Panels represent IRF-3 staining, nuclear staining, and merge plus phase-contrast images (left to right). (B) STAT1 activation in TAMs. Western blot (WB) for phospho-STAT1 expression. The cell lysates from untreated or LPS-treated (100 ng/mL) PECs and TAMs for the indicated time points were probed first in WB with phospho-STAT1 antibody and reprobed with STAT1 antibody. Equal loading is visualized by actin expression. (C) Nuclear translocation of p65 NF-κB subunit. Western blot for p65 NF-κB subunit protein in the nuclear extracts of untreated or LPS-treated PECs and TAMs for the indicated time periods. Bottom panel shows cytoplasmic levels of the same. Results are representative of 3 independent experiments.