Immunoblots of Met-α₂AP cleaved by rFAP to Asn-α₂ AP. Met-α₂AP was incubated with purified rFAP (0.2 μg) for selected times as shown at the top of each lane. At each time the incubation mixture was analyzed using 2 different antibodies. (A) rFAP diminished Met-α₂AP band intensity with time as determined by immunoblotting with an antibody specific for the N-terminal peptide of Met-α₂AP. (B) The α₂AP band intensity was not diminished when blotted by an antibody to the C-terminal region common to both Met-α₂AP and Asn-α₂AP.