The expression of iron regulatory genes in the liver of hbd and wild-type mice. Hepatic expression of hepcidin, Tfr2, hemojuvelin, and hfe mRNA in wild-type () and hbd (▪) mice on either a control or iron-deficient diet was determined by ribonuclease protection assay. The mRNA encoding ribosomal protein Arbp was used as a normalization control. (A) Representative assays for each gene. (B) Band intensities for each gene were measured by densitometry and graphed as a proportion of 36B4 expression. The data represent the mean ± SEM (n = 5-11; ^*P < .05; ^**P < .01).