CD4/CD8 DP monocytes in human peripheral blood. Human peripheral blood was obtained from 12 healthy volunteers. The cells were stained with FITC-conjugated anti-CD4 (M-T466), PE-conjugated anti-CD8 (HIT8a), and PerCP-conjugated anti-CD14 (MφP9) Abs, followed by depletion of erythrocytes, and then monocytes in region 1 (R1) were gated. The bottom panels show expression of CD4 and CD8 on CD14⁺ cells in region 2 (R2). The percentage of CD4⁺/CD8⁺ cells in CD14⁺ monocytes is shown in each panel.