Effects of GATA-2 expression on differentiation of hematopoietic cells and expression of transcription factors. GATA-2 expression commenced on day 5 and continued for the durations shown (A,C). (A) Percentages of Mac-1⁺, GPV⁺, and TER-119⁺ cells at day 9. Three independent experiments were carried out. Similar results were obtained from the experiments and representative data are shown. (B) May-Giemsa and AchE staining of cells at day 9. (C) GATA-2 was expressed from days 5 to 7. Western blotting analyses of GATA-2, PU.1, and GATA-1 proteins at day 9.