Effects of GATA-2 expression commencing at different stages of differentiation on hematopoietic cells and expression of transcription factors. The duration of GATA-2 expression was as indicated (A-B). (A) Percentages of Mac-1⁺, GPV⁺, and TER-119⁺ cells at day 9. Three independent experiments were performed. Essentially similar results were obtained from the experiments and representative data are shown. (B) May-Giemsa staining of cells at day 9. (C) RT-PCR analyses of Gata2 and PU.1 1 day after induction of GATA-2 expression. Serially diluted cDNAs (1, 1:3, and 1:9) were used for PCR. GATA-2 expression was induced on day 5 or 6 as indicated.