ALK inhibition-dependent attenuation of BaF3/NPM-ALK cell proliferation. (A-C) The BaF3 and BaF3/NPM-ALK cell lines were seeded on 96-well plates and treated with CEP compounds at indicated concentrations for 48 hours with or without IL-3. Viable cells were detected with MTS assay. The values presented are the average of relative cell numbers from 2 independent experiments with standard error. (D) For immunoblotting, the BaF3/NPM-ALK cells were treated with CEP compounds at indicated concentrations for 2 hours and lysed, and the lysates were subjected to SDS-PAGE. After transfer, membranes were blotted with either phospho-ALK or total ALK antibody.