Effect of ANXA1 on PMN interaction with HUVECs under flow. PMNs were incubated with ANXA1 for 10 minutes prior to flow over TNF-α-stimulated (10 ng/mL, 4 hours) HUVECs. PMN-endothelium interactions were quantified off-line. (A) Number of PMNs captured. (B) Number of rolling PMNs. (C) Number of firmly adherent PMNs. Data are presented as mean ± SEM of 3 to 5 independent experiments. ^*P < .05 versus control group.