Effect of peptide Ac2-26 and ANXA1 on PMN adhesion molecule expression. PMNs were incubated with either ANXA1, peptide Ac2-26, or PAF (1 μM) for 30 minutes at 37°C. Cell surface levels of l-selectin (A), CD11b (B), and PSGL-1 (C) were analyzed by flow cytometry. Data are presented as mean percent of control fluorescence ± SEM of 3 independent experiments. ^*P < .05 versus control group.