Figure 4.
Analysis of competition binding in FPR- and FPRL-1/ALX-transfected HEK-293 cells. Homoligand (peptide Ac2-26) and heteroligand (ANXA1) competition binding curves in FPR- and FPRL-1/ALX-transfected HEK cells using 50 nM [125I-Tyr]-Ac2-26 as tracer. (A,B) Displacement of tracer by unlabeled Ac2-26 at FPR and FPRL-1/ALX receptor, respectively. (C) Lack of competitive binding by ANXA1 at FPR-HEK cells. (D) Displacement of tracer by ANXA1 in FPRL-1/ALX-HEK cells. Data are presented as mean ± SEM of 4 independent experiments.