FPR- and FPRL-1/ALX-mediated early activation of ERK. (A) Representative Western blots illustrating the stimulatory effects of 10 μM peptide Ac2-26 on the short-term (2-15 minutes) activation of ERK in FPR- and FPRL-1/ALX-transfected HEK cells. The lack of effect of Ac2-26 on p38 and JNK activation is also shown. (B) As in panel A, but also using 0.5 μM ANXA1, showing 10-minute data with FPRL-1/ALX-HEK- and empty plasmid (mock)-transfected HEK cells. (C) FPRL-1/ALX-HEK cells were incubated with pertussis toxin (1 μg/mL) for 2 hours prior to 10-minute addition of 10 μM peptide Ac2-26 or 0.5 μM ANXA1. Blots are representative of at least 3 separate experiments.