Cytotoxic activity of p540-specific CTL lines. The graphs show the cytotoxic activity of 2 representative p540-specific CTL lines generated from patient nos. 11 (A) and 16 (B), respectively, against HLA-A2⁺ T2 and BJAB cells as well as HLA-A2^- RAJI cells. Effector-target ratio was 40:1. Target cells were as follows: (A) Nonpulsed T2 cells; (B) T2 cells pulsed with the p540 peptide; (C) T2 cells pulsed with the unrelated p13-32 MUC1 peptide (PAHGVTSAPDTRPAPGSTAP); (D) T2 cells pulsed with the p540 peptide plus the anti–HLA class I W6/32 mAb (10 μg/mL); (E) T2 cells pulsed with the p540 peptide plus an unrelated isotype-matched (IgG2a) mAb (10 μg/mL); (F) BJAB cells; (G) BJAB cells plus the anti–HLA class I W6/32 mAb (10μg/mL); (H) BJAB cells plus an unrelated isotype-matched (IgG2a) mAb (10 μg/mL); (I) RAJI cells; (L) RAJI cells plus the anti–HLA class I W6/32 mAb (10 μg/mL); and (M) RAJI cells plus an unrelated isotype-matched (IgG2a) mAb (10 μg/mL).