Inhibition of lactic acid production reverts the phenotypical changes in the direct MelIm MCTS coculture. MelIm MCTSs were generated in the presence or absence of oxamic acid. To exclude the possibility that oxamic acid influences DC differentiation directly, MCTSs were washed before adding the monocyte suspension. After 5 to 7 days in coculture, MCTSs were dissociated by vigorous pipetting and stained with mouse anti-human CD45 antibody in combination with antibodies against CD1a and CD14. The same staining procedure was performed with control DCs. Histograms represent CD45⁺ cells. The figure shows 1 representative experiment of 3. Open histograms represent isotype controls; filled histograms, specific staining.