American Society of Hematology

Figure 3.

$Figure 3. Incorporation of BrdU by culture-generated BM lymphoid cells stimulated or cross-stimulated in vitro with rIL-7 or scIL-7/HGFβ. BM cells from IL-7-/- mice were cultured in the presence of rIL-7 (10 ng/mL) or scIL-7/HGFβ (30 ng/mL) for 19 days. The cells were washed, cytokine-starved for 5 hours, stimulated with the homologous or heterologous cytokine for 3 hours, pulsed with BrdU, and stained with combinations of antibodies to B220, HSA, AA4.1, CD43, CD4, and BrdU. (A,C) Distribution early B-lineage subsets in each culture system. (B,D) Percentage of BrdU+ cells in each fraction of B-lineage cells. (A-B) ▪ indicates IL-7/HGFβ-generated cells stimulated with IL-7/HGFβ; ▦, IL-7-generated cells stimulated with IL-7. (C-D) ▪ indicates IL-7/HGFβ-generated cells stimulated with IL-7; ▦, IL-7-generated cells stimulated with IL-7/HGFβ. Means of duplicate samples are shown. Data are from 1 representative experiment of 2.$

Incorporation of BrdU by culture-generated BM lymphoid cells stimulated or cross-stimulated in vitro with rIL-7 or scIL-7/HGFβ. BM cells from IL-7^-/- mice were cultured in the presence of rIL-7 (10 ng/mL) or scIL-7/HGFβ (30 ng/mL) for 19 days. The cells were washed, cytokine-starved for 5 hours, stimulated with the homologous or heterologous cytokine for 3 hours, pulsed with BrdU, and stained with combinations of antibodies to B220, HSA, AA4.1, CD43, CD4, and BrdU. (A,C) Distribution early B-lineage subsets in each culture system. (B,D) Percentage of BrdU⁺ cells in each fraction of B-lineage cells. (A-B) ▪ indicates IL-7/HGFβ-generated cells stimulated with IL-7/HGFβ; ▦, IL-7-generated cells stimulated with IL-7. (C-D) ▪ indicates IL-7/HGFβ-generated cells stimulated with IL-7; ▦, IL-7-generated cells stimulated with IL-7/HGFβ. Means of duplicate samples are shown. Data are from 1 representative experiment of 2.

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