Figure 5.
Expression of the IL-7R and/or c-Met on culture-generated and normal pre-pro-B cells and pro-B cells. Images of IL-7R (green) and c-Met (red) examined by confocal microscopy show colocalization (yellow) patching and capping of IL-7R and c-Met on scIL-7/HGFβ culture-generated and enriched pre-pro-B cells from normal BM. IL-7 culture-generated cells expressed the IL-7R only. Some cytoplasmic staining is also seen in the latter cells, which were stained after the smears had been fixed. The top panels are isotype controls. Scale bars equal 10 μm.