Ability of rIL-7 or scIL-7/HGFβ to activate Jak3 and/or c-Met in mouse B-lineage bone marrow cells. B-lineage cells generated in cultures of IL-7^-/- mouse BM cells supplemented with rIL-7 or scIL-7/HGFβ were harvested, placed in cytokine-free medium for 5 hours, and then stimulated with the homologous cytokine for 10 or 30 minutes. The supernatants from lysed cells were immunoprecipitated with anti-Jak3 or antiphosphotyrosine antibody and subjected to SDS-PAGE and Western blotting using the indicated antibodies. Arrows indicate phospho-c-Met or phospho-Jak3.