In vitro–generated LDGs completely inhibit acute GVHD. (C57BL/6 × BALB/c) F1 mice were lethally irradiated, reconstituted with BMTD only, BMTD plus NWS, or NWS+LDG. (A) Percentages of surviving animals. Log rank: P = .05 between BMTD and NWS and P = .01 between NWS+LDG and NWS. (B) Weight loss for BMTD and BMTD+NWS+LDG groups. Twice a week, mice were weighed and the results plotted as the percentage of initial weight. (C) Histopathology of the small intestine. Mucosal atrophy with preservation of simple columnar epithelium of the villi are observed without significant inflammatory infiltrates in lamina propria in right and left panels. Crypts exhibit regenerative changes, with typical goblet. Original magnifications, 100 × (left panel), 200 × (right panel). Results are representative of 2 experiments with 4 to 5 mice per group.