mAb-mediated disruption of DNAM-1 interaction with PVR or Nectin-2 inhibits the NK cell-mediated lysis of autologous iDCs. Activated polyclonal NK cells from the representative donor no. 8 were analyzed for cytolytic activity against autologous iDCs either in the absence or in the presence of mAbs to the indicated molecules, used alone or in combination. The E/T ratio used was 10:1. The results are representative of 3 independent experiments; the SD of the mean of the triplicates was less than 5%.