NK92 cell line-mediated cytolysis of iDCs and mDCs: involvement of DNAM-1/ligand interaction. (A) The NK92 NK cell line was analyzed for its cytolytic activity against iDCs or mDCs derived from 3 representative donors either in the absence (▪) or in the presence () of anti-HLA class I mAb. The E/T cell ratio used was 10:1. The results are representative of 3 independent experiments; the SD of the mean of the triplicates was less than 4%. (B) NK92 was analyzed for its capability of killing iDCs or mDCs derived from the representative donor no. 2, either in the absence or in the presence of mAbs to the indicated molecules, used alone or in combination. The E/T ratio used was 10:1. The results are representative of 3 independent experiments; the SD of the mean of the triplicates was less than 5%.