Immunohistochemical analysis of Nectin-2 and PVR expression in human lymph nodes. Sections were immunostained with anti-Nectin-2 or anti-PVR mAbs (red), using the avidin-biotin-peroxidase method, and counterstained with the nuclear counterstain Gill hematoxylin (blue). (A) Scattered, positive cells for Nectin-2 are mainly distributed in the parafollicular T-cell areas of a lymph node (arrows; original magnification × 100; objective lens, 10 ×/0.25 numeric aperture [NA]). (B) Higher-power magnification of the area indicated in panel A reveals cells positive for Nectin-2 surrounding a vessel (arrows; original magnification × 250; objective lens, 25 ×/0.50 NA). (C) Several cells showing a stellate morphology and expressing Nectin-2 surround a vessel where endothelial cells are also stained (original magnification × 400; objective lens, 40 ×/0.70). (D) Few cells stained for PVR and lining a parafollicular T-cell area are visible (original magnification × 400; objective lens, 40 ×/0.70). Images were acquired using a Leica DMLB microscope. (Leica Microsystems, Wetzlar, Germany). A Canon Power Shot G5 digital camera (Canon, Tokyo, Japan) and Adobe Photoshop version 7.0.1 (Adobe Systems, San Jose, CA) were used to capture images.