Inhibitory effects of neutralizing antibodies against various adhesion molecules and chemokines on the adhesion of neutrophils, PBLs, and monocytes to and their transmigration through the ECs cocultured with SMCs in the reattachment flow area c under VSF. The EC/SMC cocultures were incubated with neutralizing antibodies (20 μg/mL) against the indicated adhesion molecules and chemokines for 1 hour prior to adhesion and transmigration assays under VSF. EC/SMC treated with IgG or without antibody treatment were used as controls. Purified neutrophils, PBLs, and monocytes were perfused over the EC/SMC for 20 minutes, and the adhesion and transmigration of the WBCs in the reattachment flow area c were determined, as described in “Materials and methods.” Data represent percentage of inhibition, which was calculated from the number densities (in cells/mm²) of adherent (▪) or transmigrated (▦) WBCs with antibody treatment (C_E) and without antibody treatment (C_C): % inhibition = 100 [(C_C - C_E)/C_C]. Data are expressed as mean ± SEM, n = 4. ^*P < .05 versus EC/SMC treated with control IgG or without antibody treatment.