Figure 2.
Tax transcriptional activation of CREB/ATF- and NFκB-dependent reporter genes. (A) 293T cells (2 × 105) were cotransfected with 2 μg Tax expression vector or a negative control, 0.02 μg TK-Renilla, and 0.1 μg LTR-1–Luc. Cell lysates were harvested 48 hours after transfection and subjected to a dual luciferase assay. The histogram presents the average fold activation over control values for 3 independent experiments, and error bars denote SDs. (B) Jurkat T cells (4 × 106) were cotransfected with 3 μg Tax expression vector or a negative control, 0.05 μg TK-Renilla and 0.25 μg κB-Luc, and luciferase activity was measured as presented in panel A.
