Figure 1.
Integrin α6 is expressed in mouse BM HSCs. BM cells were stained and gated for Lin– and 7AAD– cells (left panels). Lin–/7AAD– cells were gated for Sca-1– C-Kit+ clonogenic progenitors and the more primitive Sca-1+ C-Kit+ (LSK) cells; 93% and 99% of the cells expressed integrin α6 receptor (α6), respectively. The top and middle right panels show staining with the integrin α6 antibody (open histograms) and isotype control antibody (gray histograms). The bottom right panel shows expression of integrin α6 receptor in LSKCD34– and LSKCD34+ cells. The vertical and horizontal bars were set on the basis of isotype-matched negative control profiles (> 99% negative). Shown is one representative analysis of 2.