Chemokine receptor induction on OT-II cells following activation with splenic or MLN DCs in vitro. CFSE-labeled OT-II cells were cultured with OVA-pulsed splenic or MLN DCs, and chemokine receptor expression assessed on responding OT-II cells 3.5 days later. Plots are from one representative experiment of 3 to 5 performed using pooled DCs from 5 to 10 mice/experiment. Numbers represent mean (SEM) of 3 to 5 experiments.