In vivo gene marking levels derived from retroviral transduction of AMD3100-mobilized CD34⁺ cells. Presence of vector in PBMCs and GRANs at various times (in months) after transplantation of retrovirally transduced AMD3100-mobilized CD34⁺ cells in rhesus macaques was detected by real-time PCR. Results are presented for animal RC909 (A) and RQ2851 (B). Gene marking levels were also determined in B and T cells 32 months (RC909; B cells, 0.2%; T cells, 0.5%) or 28 months (RQ2851; B cells, 1%; T cells, 4%) after transplantation.