Cell-cycle analysis. CD34⁺ cells were analyzed using flow cytometry after staining with acridine orange to measure the G₀, G₁, S, and G₂/M contents of each cell source (AMD3100-mobilized PB cells, G-CSF-mobilized PB cells, and BM) cells). (A) Representative experiment (animal RQ3636). The percentage of cells in each phase of the cell cycle is shown in the accompanying table below each flow cytometry panel. (B) Summary of the percentage of CD34⁺ cells in each phase of the cell cycle. The P values comparing G-CSF-mobilized or BM CD34⁺ cells with AMD3100-mobilized CD34⁺ cells are shown in the accompanying table below the summary chart.