Anti-CD94 mAb blocks the expansion of NKG2C⁺ NK cells stimulated by HCMV-infected fibroblasts. (A) PBLs from an HCMV⁺ donor were cultured with mock- and Towne-infected (MOI = 1) MRC-5 cells. Either F(ab′)2 anti-CD94 or -CD56 mAbs (10 μg/mL) were added at day 0. Flow cytometry analysis with anti-CD3, -NKG2C, and -NKG2A mAbs was carried out at day 10. (B) The differential effects in the numbers of recovered cells in the presence of the anti-CD94 mAb added at day 0 or day 4 are compared. The data are representative of 5 experiments performed.